Journal: Journal of Inflammation Research

Article Title: Pan-Cancer Screening and Validation of CALU’s Role in EMT Regulation and Tumor Microenvironment in Triple-Negative Breast Cancer

doi: 10.2147/JIR.S477846

Figure Lengend Snippet: Validation of CALU, EMT marker genes, CD3 and α-SMA in breast cancer samples. ( A ) IHC staining scores for CALU and EMT markers from 8 TNBC patients.( B and C ) IHC staining results of CALU, E-cadherin, N-cadherin, Vimentin and Snail1 in TNBC tissues. ( D ) IF staining results of CALU and CD3 in breast cancer tissue. ( E ) IF staining results of CALU and α-SMA in breast cancer tissue.**p< 0.01; ***p< 0.001.

Article Snippet: Primary antibodies, including CALU (1:100, Rabbit, PTG, 17804-1-AP), α-SMA (1:400, Mouse, Boster BM0002), and CD3 (1:200, Mouse, PTG, 60181-1-Ig), were diluted with 5% BSA and applied to the sections, which were incubated overnight at 4°C.

Techniques: Biomarker Discovery, Marker, Immunohistochemistry, Staining